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This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
大年初二，我们从老家出发前往川东访古，原本人烟稀少的高速路，竟也车水马龙。我开启了车载的自适应巡航功能——在此之前，我驾驶时仅使用过定速巡航，即按设定速度行驶，无须踩“油门”。